TABLE 2.
Predicted locations and fusion promotion activities of G glycoprotein mutantsa
| G mutant | Predicted location | % WT fusion (n) |
|---|---|---|
| E254A HeV G | β1S3 | 44.2 (2) |
| D257A HeV G | β1S3 | 27.1 (2) |
| D260A HeV G | β1L34 | 35.9 (2) |
| K261A HeV G | β1L34 | 81.8 (2) |
| D326A HeV G | β2S4 | 102.6 (2) |
| D329A HeV G | β2S4 | 108.5 (2) |
| G439A HeV G | β3/β4 loop | 94.0 (2) |
| K443A HeV G | β4S1 | 10.8 (2) |
| N446A HeV G | β4S1 | 83.5 (2) |
| G449A HeV G | β4L12 | 80.4 (3) |
| K465A HeV G | β4S3 | 15.3 (3) |
| D468A HeV G | β4S3 | 40.8 (3) |
| D470A HeV G | β4S3 | 116.7 (3) |
| E553, D554A HeV G | β5/β6 loop | 52.1 (3) |
| K560A HeV G | β5/β6 loop | 58.6 (3) |
| D564A HeV G | β6S1 | 134.3 (3) |
| E569A HeV G | β6S1 | 90.8 (3) |
a
The putative locations of the G substitution mutants are listed based on a proposed structure (38, 40), along with their average fusion promotion activities. Receptor-positive 293T cells were employed as the target cell population. The percent WT fusion promotion activity for each mutant was calculated, and the results of n (as indicated in parentheses) independent experiments were averaged.