FIG. 4.

Phylogenetic analysis of HAdV-52 is based on the predicted amino acid sequences of pTP (A), pIIIa (B), and penton base (C) from selected adenoviruses. Numbers denote human adenovirus serotypes. HAdV-52 (in bold) shows the new isolate. Simian adenoviruses are designated by the word simian and their serotype number. Note that simian adenoviruses 1 and 3 are from Old World monkeys, while SAdV-21 to -25 were isolated from chimpanzees. All of these trees are unrooted. SAdV-3 was chosen as an outgroup, as earlier studies suggested it to be the most ancient from the fully sequenced primate adenoviruses (21, 22). Official human adenovirus species are designated by the letter used for their subsequent lettering, e.g., F for human adenovirus F. Simian adenovirus A is shown by its abbreviated name, SAdV-A. The newly proposed HAdV species (containing a monkey and a human AdV type) is designated “proposed G” (in bold). Bootstrap values (for 100 data sets) are shown for the accepted and the presently proposed species.