Secondary structure of isolated tRNAs. The optimal secondary structure patterns are shown for the type 7-1, 7-2, 8, 9, and 10 tRNAs, which were isolated in these experiments. Types 7-1 and 7-2 are shown with the covariation data from the D-stem of these tRNAs. None of these sequences can be folded into the canonical cloverleaf structure.