Table 2.
PNA-targeted mutagenesis of a chromosomal gene in mouse cells
| Treatment | supFG1 gene, mutants/total | Frequency, ×10−5 | cII gene, mutants/total | Frequency, ×10−5 |
|---|---|---|---|---|
| No oligo | 7/74,093 | 9 | 2/51,868 | 4 |
| PN-8 | 52/62,359 | 83 | ND | ND |
| PN-10 | 62/71,437 | 86 | 4/45,288 | 9 |
| CP-10 | 10/73,185 | 14 | 4/49,846 | 8 |
| RP-10 | 14/67,551 | 21 | ND | ND |
The PNAs were introduced into mouse 3340 cells via SLO-mediated permeabilization. After 3–4 days, the cells were harvested for DNA preparation and rescue of the chromosomal shuttle vector. In each case, the frequency of mutant phage carrying mutations in the supFG1 and cII reporter genes was calculated. ND, not determined.