Figure 5.

Competition binding (dbd)Fos–Jun to oppositely phased hcgDNA15 and hcgDNA19 isomers in buffers containing 2 mM Mg²⁺: kinetic order of addition effect. The DNA substrate added first was incubated with limiting amounts of (dbd)Fos–Jun for 2 min, followed by incubation with the DNA substrate added second, for the times indicated in the figure. Note that the geometric means of the measured K_rel values, which are constant at the different time points, represent the equilibrium values. The K_rel values for both isomers are similar in magnitude indicating that low salt does not promote DNA bending by (dbd)Fos–Jun.