Table 1.

Relative binding affinities (K_rel) of (dbd)Fos–Jun for linear and circular DNAs: Effect of DNA length and DNA phasing

DNA	DNA length, bp	Phasing*	Krel (Klinear/Kcircle)	[J(−fj)/J(+fj)]†
hcgDNA11	158	11	1.3
hcgDNA15	158	15	1.4
hcgDNA19	158	19	1.2
hcgDNA35	158	35	1.7
hcgDNA39	158	39	1.6
hcgDNA43	158	43	1.5
hcgDNA35	154	35	1.4
hcgDNA39	154	39	1.3
hcgDNA43	154	43	1.4
yeastDNA35	158	35	1.4	0.80
yeastDNA39	158	39	1.3	1.10
yeastDNA43	158	43	1.2	0.71

The K_rel values are obtained in buffers containing 5 mM Mg²⁺ and represent averages of at least three experiments. 

^*The phasing represents the distance between the centers of the AP-1 site and the closest A-tract sequence. 

^†The term [J(−fj)/J(+fj)] is the ratio of the J factors (cyclization probabilities) in the absence and presence of Fos–Jun, calculated from the data in ref. 9.