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. 1998 Feb 17;95(4):1416–1420. doi: 10.1073/pnas.95.4.1416

Figure 5.

Figure 5

Michaelis–Menten plot. Labeled DNA at a final concentration of 0.8 nM was mixed with different amounts (0–130 nM) of unlabeled DNA and incubated with 160 pM EcoRI in the reaction buffer at 27°C. The reactions were monitored on-line and the initial rates v were derived by linear regression of data points of the first 5–20 min. (Inset) Calculations from an Eadie–Hofstee plot lead to a KM value of 14 ± 1 nM and vmax of 0.74 ± 0.03 nM min−1.

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