Figure 4.

Rapamycin inhibit growth of the ScFKBP expressing lines and reduce polysome accumulation. WT:control; 25c: transgenic line expressing ScFKBP12. A. Effect of rapamycin on growth expressed as fresh weight per mg of seeds. Seeds were sown in liquid medium, incubated 48 h at 4°C, germination and grown under constant light during 10 days. Rapamycin was added at 10 μg/ml. B. Polysome profile from plantlets described in A. Polysomes were displayed on sucrose gradients and profiles recorded at 260 nm.