Table 1.

TCSPC data for ECFP and CY24. a₁ and a₂ are the exponential coefficients (%) for the t₁ and t₂ decay times, respectively. n indicates the number of independent experiments performed (i.e. the number of cells examined). Data are derived from whole cell regions of interest, and are expressed as mean ± SD.

Group	a1 (%)	τ1 (ps)	a2 (%)	τ2 (ps)	τ̄ (ps)	n
ECFP	38 ± 5	420 ± 120	62 ± 5	2190 ± 240	1570 ± 60	11
CY24	42 ± 5	340 ± 70	59 ± 5	1980 ± 50	1280 ± 120	8

λ_excitation = 800 nm TPE, λ_emission = 435-485 nm BP.