Table 2.

TCSPC data for FM1-43 and FM1-43/FM4-64 co-labelling. The symbols are as for Table 1. The data were acquired from the same FM1-43 cells before and after counter-labelling with FM4-64 (see Methods).

Group	a1 (%)	τ1 (ps)	a2 (%)	τ2 (ps)	$\stackrel{‒}{\tau }$ (ps)	n
FM1-43	48 ± 5	261 ± 106	50 ± 4	1796 ± 135	989 ± 133	3
FM1-43/4-64	73 ± 2	163 ± 64	26 ± 5	1291 ± 234	496 ± 72	3

λ_excitation = 800 nm TPE, λ_emission = 500-550 nm BP.