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Transcriptional activation of pool II L by mammalian cell extract. The in vitro VSV transcription reaction was carried out as described in Fig. 1. The cytoplasmic extract of Sf21 or HeLa cells (10 μg), rabbit reticulocyte lysate (5 μl), and βγ purified from A. salina (25 ng) were added to the transcription reactions. The transcription products were analyzed as in Fig. 1.
