Abstract
Lipase-positive cells were separated from suspensions of pancreas cells both by velocity sedimentation and by isopycnic sedimentation. Lipase-positive cells were 51.0 ± 9.6% of the disaggregated pancreas cells in the starting sample suspension. The purest gradient fractions from experiments using velocity sedimentation for cell separation contained a mean of 98.8 ± 0.6% lipase-positive cells. Cell separation using isopycnic centrifugation was less effective than cell separation using velocity sedimentation and resulted in a mean purity of only 89.8 ± 6.8% lipase-positive cells. Lipase positivity was assessed using the Gomori technic with the conditions for fixation slightly modified to be suitable for disaggregated cells.
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