Skip to main content
NCBI home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1973 Oct;73(1):147–156.

Peroxidase Arthritis

III. Sequential Changes in Cellular Composition of the Developing Inflammatory Infiltrate

Richard C Graham Jr, Sarajayne Limpert Shannon
PMCID: PMC1904038  PMID: 4749212

Abstract

Repeated daily injections of horseradish peroxidase into rabbit knee joints lead to a characteristic sequence of cellular events. Early transient infiltration with polymorphonuclear leukocytes was followed by a progressive increase in the number of macrophages. This appeared to represent, at least in part, a consequence of increased emigration of monocytes. Subsequently, increasing numbers of lymphocytes appeared. Many of the lymphocytes had frequent cytoplasmic polyribosomes. By the eighth or ninth day, immunoblasts, plasmablasts and immature plasma cells were observed; cytochemical evidence of local specific antibody synthesis first was demonstrated in such cells at this stage. These results demonstrate the sequential cellular events in an immunologic inflammatory response to local antigen. Questions remain concerning the stimulus for early monocyte emigration and macrophage accumulation, and the site of initial sensitization of lymphocytes; these problems are discussed.

Full text

PDF
147

Images in this article

155Fig 4
on p.155
155Fig 5
on p.155
156Fig 6
on p.156
156Fig 7
on p.156
153Fig 1
on p.153
153Fig 2
on p.153
153Fig 3
on p.153

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BALL J., CHAPMAN J. A., MUIRDEN K. D. THE UPTAKE OF IRON IN RABBIT SYNOVIAL TISSUE FOLLOWING INTRA-ARTICULAR INJECTION OF IRON DEXTRAN. A LIGHT AND ELECTRON MICROSCOPE STUDY. J Cell Biol. 1964 Aug;22:351–364. doi: 10.1083/jcb.22.2.351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CHRISTIAN C. L. RHEUMATOID ARTHRITIS--ETIOLOGIC CONSIDERATIONS. Arthritis Rheum. 1964 Oct;7:455–466. doi: 10.1002/art.1780070502. [DOI] [PubMed] [Google Scholar]
  3. COCHRANE W., DAVIES D. V., PALFREY A. J. ABSORPTIVE FUNCTIONS OF THE SYNOVIAL MEMBRANE. Ann Rheum Dis. 1965 Jan;24:2–15. doi: 10.1136/ard.24.1.2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cohn Z. A. The structure and function of monocytes and macrophages. Adv Immunol. 1968;9:163–214. doi: 10.1016/s0065-2776(08)60443-5. [DOI] [PubMed] [Google Scholar]
  5. David J. R. Delayed hypersensitivity in vitro: its mediation by cell-free substances formed by lymphoid cell-antigen interaction. Proc Natl Acad Sci U S A. 1966 Jul;56(1):72–77. doi: 10.1073/pnas.56.1.72. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  7. Graham R. C., Jr, Shannon S. L. Peroxidase arthritis. I. An immunologically mediated inflammatory response with ultrastructural cytochemical localization of antigen and specific antibody. Am J Pathol. 1972 Apr;67(1):69–94. [PMC free article] [PubMed] [Google Scholar]
  8. Graham R. C., Jr, Shannon S. L. Peroxidase arthritis. II. Lymphoid cell-endothelial interactions during a developing immunologic inflammatory response. Am J Pathol. 1972 Oct;69(1):7–24. [PMC free article] [PubMed] [Google Scholar]
  9. HOLLINGSWORTH J. W. CELLULAR REACTIONS TO SOLUBLE FOREIGN MATERIALS IN THE RABBIT KNEE JOINT. Yale J Biol Med. 1965 Feb;37:300–312. [PMC free article] [PubMed] [Google Scholar]
  10. Jasin H. E., Ziff M. Immunoglobulin and specific antibody synthesis in a chronic inflammatory focus: antigen-induced synovitis. J Immunol. 1969 Feb;102(2):355–369. [PubMed] [Google Scholar]
  11. Leduc E. H., Avrameas S., Bouteille M. Ultrastructural localization of antibody in differentiating plasma cells. J Exp Med. 1968 Jan 1;127(1):109–118. doi: 10.1084/jem.127.1.109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Smith C. B., Ward J. R. "Chronic infectious arthritis"--role of mycoplasmas. J Infect Dis. 1971 Mar;123(3):313–315. doi: 10.1093/infdis/123.3.313. [DOI] [PubMed] [Google Scholar]
  13. Stavitsky A. B., Folds J. D. The differential localization of antibody synthesis and of immunologic memory in lymph nodes draining and not draining the site of primary immunization with hemocyanin. J Immunol. 1972 Jan;108(1):152–160. [PubMed] [Google Scholar]
  14. Williams M. H., Brostoff J., Roitt I. M. Possible role of Mycoplasma fermentans in pathogenesis of rheumatoid arthritis. Lancet. 1970 Aug 8;2(7667):277–280. doi: 10.1016/s0140-6736(70)91328-0. [DOI] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES