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. 2006 Jun 28;34(11):e78. doi: 10.1093/nar/gkl424

Figure 2.

Figure 2

Agarose gels of PCR products following (a) p16 MSP, (b) p16 MEP, and (c) cyclin A1 MEP. U, PCR using MSP primers specific for unmethylated DNA; M, PCR using methylation-specific MSP primers; A and B, two independent MEP assays. Rows 1–4 (top to bottom) correlate with rows 1–4 in dilution matrix Table 1; i.e. row 1, no dilution of starting M:U concentrations; row 2, 1/10 dilutions, etc. Lanes 1–8 correlate with columns 1–8 in dilution matrix Table 2; i.e. lane 1, M:U ratio of 1:1; lane 2, M:U ratio of 1:5, etc.