Figure 5.

Western blots of total protein lysates from young and old mouse ovaries. (a) Proteins with reduced content in old (o) ovaries compared with young ovaries (y); (b) proteins with higher level in young ovaries; and (c) no change in protein content. Proteins were visualized with protein-specific antibodies as indicated at the left of the panels. The size (kDa) of the protein marker is indicated at the right of the panels. All lanes received 100 μg of total ovarian lysate. The immunological reactions are visualized by HSP-coupled secondary antibodies.