Table 1.
Genes with age-dependent changes in mRNA and protein levels in mouse ovaries
| Accession | Gene | RNA | Protein | ||
|---|---|---|---|---|---|
| Level | Method | Level | Method | ||
| Steroids and growth factors | |||||
| TC188770 | OB Leptin | D | 2,3 | NC | 4 |
| M13177 | Transforming growth factor B | D | 2,3 | NC | 4 |
| NM_023135 | Estrogen sulfotransferase | U | 1 | D | 4,5 |
| D000659 | Aromatase | U | 1 | NC | 4,5 |
| NM_008829 | Progesterone receptor | U | 1 | NC | 4 |
| Transcription factors | |||||
| NM_009537 | Yin Yang 1 | D | 2,3 | D | 4,5* |
| AF022363 | SP1 | D | 3 | D | 4,5* |
| M57999 | Nuclear factor κb | U | 2,3 | U | 5** |
| X57528 | Retinoic acid receptor α | U | 2,3 | U | 5** |
| BC062915 | GATA-3 | U | 1,3 | D | 5 |
| NM_011144 | Peroxisome proliferatory-activated receptor α | U | 1,2,3 | NC | 4,5 |
| Cell signaling | |||||
| BC037598 | GDP dissociation inhibitor 1 | D | 1,3 | D | 4,5* |
| AA152708 | Jagged1 | D | 1,2,3 | NC | 4,5 |
| NM_008716 | Notch 3 | D | 1,3 | NC | 4,5 |
| UO7951 | GDP dissociation inhibitor 2 | U | 1 | U | 4** |
| NM_153400 | Purinergic receptor P2X2 | U | 1 | U | 4,5** |
| BC031297 | Janus kinase 1 | U | 2,3 | D | 4,5 |
| Stress-induced | |||||
| NM_010442 | Heme oxygenase 1 | U | 2,3 | U | 4,5** |
| U73744 | Heat shock cognate 70 | U | 2,3 | U | 4** |
| M35021 | Heat shock protein 70 | U | 3 | NC | 4 |
| Other | |||||
| L22550 | Desmin | D | 1,2,3 | D | 5* |
| X82786 | Ki-67 cell proliferation antigen | D | 1 | D | 5* |
| NM_016960 | Small inducible cytokine subfamily A20 | U | 1 | U | 5** |
| AF051911 | Telomerase reverse transcriptase | U | 2,3 | U | 5** |
| BC100341 | Ubiquitin | U | 2,3 | D | 4,5 |
Genes with age-dependent differential expression at the level of mRNA were identified by (1) mouse LabChip array; (2) virtual northern analysis; and (3) DNA aging-specific gene array. Genes included above were confirmed to change with aging >3-fold in duplicate assays. These genes were then screened for differential protein content by western blotting (4) and (or) localization by immunofluorescence (5). From left to right, the table columns are the GenBank accession number, encoded gene, upregulation (U) or downregulation (D) with aging, methodology used to quantify and compare mRNA levels and protein content. Single asterisk marks genes downregulated both at the level of RNA and protein and double asterisk marks genes upregulated both at the level of RNA and protein.