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Materials for fluorescence labeling. (A) The structure of a fluorescent puromycin derivative. A fluorophore (Cy5 or RhG) and iminobiotin were chemically conjugated to puromycin through a linker. (B) DNA construction for fluorescence labeling of proteins. Template DNA consists of SP6 promoter, Omega sequence and an open reading frame (ORF) with a T7·tag at the N-terminus and a polyhistidine tag at the C-terminus, followed by a XhoI restriction enzyme site.
