Table 1.
Concentrations of fluorescent proteins and apparent Kd values determined using FCCS in this study
| Cy5-labeled protein (nM) | RhG-labeled protein (nM) | Addition | Kd (nM) |
|---|---|---|---|
| Fos (18.4) | Fos (18.0) | — | ND |
| Fos (19.2) | Fos (17.9) | AP-1 DNA | 3720 |
| Jun (8.1) | Jun (15.2) | — | 270 |
| Jun (7.8) | Jun (14.6) | AP-1 DNA | 190 |
| Jun (4.8) | Fos (12.3) | — | 69 |
| Jun (4.5) | Fos (11.5) | AP-1 DNA | 45 |
| Rab3A (4.8) | CaM (8.1) | — | ND |
| Rab3A (4.5) | CaM (7.8) | EGTA | ND |
| Caldesmon (6.1) | CaM (8.3) | — | 500 |
| Caldesmon (5.7) | CaM (7.5) | EGTA | 2300 |
| Calcineurin (11.2) | CaM (7.8) | — | 160 |
| Calcineurin (11.5) | CaM (7.9) | EGTA | 2200 |
| M33 (5.6) | Bmi1 (7.8) | — | 92 |
| M33 (4.6) | RYBP (5.3) | — | 70 |
| M33 (5.2) | Ring1A (7.7) | — | 51 |
| Ring1A (4.6) | RYBP (16.3) | — | 74 |
| Bmi1 (4.4) | RYBP (14.7) | — | 2300 |
| Bmi1 (4.1) | Ring1A (5.5) | — | 2000 |
ND; not determined.