Fig. 2.

Chronic exposure to Taxol reduces NCS-1 levels. (A) Representative Western blot, probed for NCS-1, shows decreased immunoreactivity after Taxol treatment, for this protein but not the loading control (β-actin). (B) Normalized NCS-1 immunoreactivity is significantly reduced in Taxol-treated cells (800 ng/ml, 6 h). (C) shRNA-mediated NCS-1 knockdown (dashed line) reduces the Ca²⁺ response to ATP similar to Taxol treatment. (D) NCS-1 levels recover to baseline 26 h after treatment with Taxol (800 ng/ml, 6 h). (E) Representative Ca²⁺ response of cells treated for 6 h with vehicle (solid line) or Taxol (800 ng/ml, 6 h; dashed line) followed by a 26-h recovery period. ∗, P < 0.05.