Table 1.
Terminal fat pad weights in Il18−/− and WT mice
| Type of adipose tissue | Female |
Male |
||||||
|---|---|---|---|---|---|---|---|---|
| Young adult |
Mature |
Young adult |
Mature |
|||||
| WT (n = 7) | Il18−/− (n = 8) | WT (n = 5) | Il18−/− (n = 10) | WT (n = 7) | Il18−/−(n = 11) | WT (n = 6) | Il18−/− (n = 6) | |
| Raw fat mass, g | ||||||||
| Brown* | 0.06 ± 0.01 | 0.08 ± 0.01 | 0.08 ± 0.01 | 0.10 ± 0.01 | 0.07 ± 0.01 | 0.09 ± 0.01 | 0.12 ± 0.01 | 0.18 ± 0.02 |
| Inguinal† | 0.29 ± 0.04 | 1.22 ± 0.24 | 0.55 ± 0.04 | 1.71 ± 0.25 | 0.62 ± 0.12 | 1.69 ± 0.09 | 0.94 ± 0.17 | 1.93 ± 0.20 |
| Subcutaneous† | 0.17 ± 0.03 | 0.49 ± 0.08 | 0.33 ± 0.02 | 0.64 ± 0.08 | 0.32 ± 0.06 | 0.96 ± 0.09 | 0.46 ± 0.06 | 0.99 ± 0.10 |
| Gonadal† | 0.21 ± 0.04 | 0.89 ± 0.20 | 0.46 ± 0.05 | 2.02 ± 0.34 | 0.65 ± 0.12 | 1.59 ± 0.09 | 0.91 ± 0.15 | 2.05 ± 0.17 |
| Retroperitoneal† | 0.03 ± 0.01 | 0.15 ± 0.04 | 0.08 ± 0.01 | 0.32 ± 0.06 | 0.17 ± 0.04 | 0.45 ± 0.03 | 0.17 ± 0.03 | 0.51 ± 0.07 |
| Mesenteric† | 0.11 ± 0.01 | 0.24 ± 0.03 | 0.25 ± 0.02 | 0.52 ± 0.08 | 0.15 ± 0.02 | 0.62 ± 0.06 | 0.32 ± 0.05 | 0.67 ± 0.06 |
| Relative fat mass, % | ||||||||
| Brown | 0.4 ± 0.03 | 0.4 ± 0.03 | 0.5 ± 0.03 | 0.4 ± 0.03 | 0.3 ± 0.02 | 0.4 ± 0.02 | 0.5 ± 0.03 | 0.6 ± 0.05 |
| Inguinal† | 1.9 ± 0.2 | 5.9 ± 1.1 | 3.2 ± 0.1 | 7.0 ± 0.8 | 2.9 ± 0.5 | 6.7 ± 0.2 | 3.8 ± 0.5 | 6.5 ± 0.4 |
| Subcutaneous† | 1.1 ± 0.1 | 2.5 ± 0.4 | 1.9 ± 0.1 | 2.7 ± 0.2 | 1.5 ± 0.2 | 3.7 ± 0.3 | 1.9 ± 0.2 | 3.3 ± 0.2 |
| Gonadal† | 1.4 ± 0.2 | 4.2 ± 0.9 | 2.7 ± 0.2 | 8.2 ± 1.2 | 3.0 ± 0.5 | 6.3 ± 0.2 | 3.7 ± 0.5 | 6.9 ± 0.3 |
| Retroperitoneal† | 0.2 ± 0.04 | 0.7 ± 0.2 | 0.4 ± 0.05 | 1.3 ± 0.2 | 0.8 ± 0.2 | 1.8 ± 0.1 | 0.7 ± 0.1 | 1.7 ± 0.2 |
| Mesenteric† | 0.7 ± 0.1 | 1.2 ± 0.1 | 1.5 ± 0.1 | 2.1 ± 0.3 | 0.7 ± 0.1 | 2.4 ± 0.2 | 1.3 ± 0.2 | 2.3 ± 0.2 |
| All white fat pads† | 5.4 ± 0.7 | 14.5 ± 2.5 | 9.7 ± 0.5 | 21.3 ± 2.5 | 8.9 ± 1.4 | 20.8 ± 0.8 | 11.4 ± 1.4 | 20.7 ± 1.1 |
Data express mean± SEM. Carcasses from Il18−/− mice were fattier. Relative mass (% carcass weight) of brown fat did not differ per genotype, P > 0.11. In contrast, each white fat pad was disproportionately larger in male and female Il18−/− mice as compared with WT controls by young adulthood (18 weeks), continuing into maturity (males, 37 weeks; females, 30 weeks of age).
*Values for male and female Il18−/− were greater than respective WT mice (P < 0.05).
†Male, but not female, Il18−/− were greater than respective WT mice (P < 0.05) (Student's and Welch's t tests for groups with equal and unequal variance, respectively).