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Bcl-2 maintains Δψ and ΔpH in the presence of KCN without affecting O2 consumption. Mitochondria (1 mg of protein per ml) isolated from livers were treated with KCN at 10 μM (A and B) or at the indicated concentrations (C), Δψ was assessed by Rh123 uptake (A), ΔpH was determined with [3H]acetate (B), and the respiration rate was measured with an O2 electrode (C).
