Table 2.
Chromosome locations and putative annotations of the PLUG markers
| Wheat chromosome | |||||
| Marker no. | Type | undigested, 1% agarose | Hae III-digest, 4% agarose | Taq I-digest, 4% agarose | Annotation of orthologous rice gene (Pseudomolecules ver. 4) |
| 1 | III | 3A, 3B, 3D | Phospholipase/Carboxylesterase family protein | ||
| 2 | III | 3A, 3B | 3A, 3B | Elicitor-responsive protein 1, putative | |
| 3 | II | 6B | 6B | 6A, 6B, 6D | GTP-binding protein, putative |
| 4 | III | 6B, 6D | expressed protein | ||
| 5 | III | 5A, 4D | 5A, 4D | CIPK-like protein 1, putative | |
| 6 | III | magnesium transporter CorA-like family protein, putative | |||
| 7 | III | Cysteine synthase, chloroplast precursor, putative | |||
| 8 | III | 2A, 2B, 2D | 2B | RNA recognition motif family protein | |
| 9 | III | 1A, 1B, 1D | AML6, putative | ||
| 10 | I | 1A, 1B, 1D | 1A, 1B, 1D | 1A | chlorophyll synthase, ChlG family protein |
| 11 | III | 7A, 7D | senescence-associated protein, putative | ||
| 12 | I | 7A, 7B, 7D | 7A, 7B, 7D | 7A, 7B, 7D | Polyprenyl synthetase family protein |
| 13 | III | 3A, 5A, 5D | Phosphatidylinositol N-acetylglucosaminyltransferase subunit A, putative | ||
| 14 | III | COP9 signalosome complex subunit 7, putative | |||
| 15 | III | 7A | 7B, 7D | MSP domain containing protein | |
| 16 | II | 7B | 7B | expressed protein | |
| 17 | III | 7A | BadF/BadG/BcrA/BcrD ATPase family protein | ||
| 18 | II | 5A | 5A, 5B | 5A, 5B, 5D | Triosephosphate isomerase, chloroplast precursor, putative |
| 19 | II | 1A | 1B, 1D | 1A, 1B, 1D | ATP synthase gamma chain, mitochondrial precursor, putative |
| 20 | III | 1A | 1A | PRP19/PSO4 homolog, putative | |
| 21 | III | Ubiquinol-cytochrome c reductase complex 7.8 kDa protein, putative | |||
| 22 | III | Small GTP-binding protein domain containing protein | |||
| 23 | III | 5A | 5B | Aspartyl aminopeptidase, putative | |
| 24 | II | 5B | 5A, 5B, 5D | 5A, 5B | expressed protein |
Types I, II and III indicate that 1% agarose gel electrophoresis of PCR products resulted in the separation of three, two, or single bands, respectively. PLUG markers were assigned to chromosomes by electrophoresis on 1% agarose gels, or by electrophoresis of HaeIII- or TaqI-digested fragments on 4% agarose gels. The table also shows the annotations of TaEST-LUGs that were used for marker development.