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. 2007 May 25;8:44. doi: 10.1186/1471-2199-8-44

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Copyright © 2007 Deibler et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Hin-mediated effect on plasmid replication. (A) Plasmid DNA was isolated from strains C600 or parCts containing the plasmids indicated. The DNA was linearized with HindIII, which cuts all the plasmids once, including pHIN. Shown is a representative ethidium bromide-stained agarose gel from an experiment performed at 42°C. Given below the gel image are the mean plasmid level values of three independent experiments with standard deviations. (B) Plasmid DNA was isolated 50 min. after IPTG induction of Hin, nicked, displayed by high-resolution gel electrophoresis and visualized by Southern blotting. Shown is an autoradiogram. All lanes contain plasmid DNA from the pHIN-harboring parCts cells with pBR, pREC or pKNOT. The positions of nicked dimer (), linear dimer () catenanes (e.g., ) are indicated.

Inline graphic — Hin-mediated effect on plasmid replication. (A) Plasmid DNA was isolated from strains C600 or parCts containing the plasmids indicated. The DNA was linearized with HindIII, which cuts all the plasmids once, including pHIN. Shown is a representative ethidium bromide-stained agarose gel from an experiment performed at 42°C. Given below the gel image are the mean plasmid level values of three independent experiments with standard deviations. (B) Plasmid DNA was isolated 50 min. after IPTG induction of Hin, nicked, displayed by high-resolution gel electrophoresis and visualized by Southern blotting. Shown is an autoradiogram. All lanes contain plasmid DNA from the pHIN-harboring parCts cells with pBR, pREC or pKNOT. The positions of nicked dimer (), linear dimer () catenanes (e.g., ) are indicated.