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Potential models for the Hin-mediated effect. Plasmid pKNOT is recombined by Hin to knot the DNA (a single line represents the central axis of the double helix). In the roadblock model, the knot (or possibly Hin bound to or cleaving DNA) is impassable and stalls polymerase. Alternatively, in the breakage model, knots may break DNA as a result of forces on the plasmid.
