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. 2007 Jul 11;2(7):e605. doi: 10.1371/journal.pone.0000605

Figure 2. Transformed cells are more sensitive to stress than normal primary keratinocytes.

Figure 2

A. Cells were serum starved (white) or untreated (black) for 48 h. After treatment, cells were fixed, stained with propidium iodide and analyzed by flow cytometry. The sub-G1 fraction of the cells was quantified as a measure of cell death. Error bars represent the standard error of three independent experiments. B. Representative western blot of cells after 24 h starvation. PARP cleavage reflects caspase activation during apoptosis. Tubulin serves as a loading control. C. Immuno-staining of cytochrome C after 12 h serum starvation (Bar = 15 µm). D. Cells were treated with 20 µM Cisplatin for 48 h, fixed and analyzed as in A. Error bars represent the standard error of three independent experiments.