Table 2.
RT-PCR results on EBV LF3 transcripts in tumour biopsies and cell linesa
| Promoter-related | (P1) | (P2) | (P2) | (P3) | (P4) | (P4) | (P4) | (P4) |
|---|---|---|---|---|---|---|---|---|
| Primers | Ia-2T + Ia-3B | Ia-2T + Ia-4B | Ia-2T + Ia-5B | Ia-2T + Ia-6B | Ia-6T + Ia-8B | Ia-6T + Ia-9B | Ia-6T + Ia-10B | Ia-6T + Ia-11B |
| Product | ||||||||
| Tumours and cell lines | 143198– 143403b | 143198– 143647 | 143198– 143783 | 143198– 143881 | 144246– 144400 | 144246– 144669 | 144246– 144704 | 144246– 144791 |
| C15 | ++c | + | + | + | + | ND | + | − |
| C17 | + | + | + | + | − | ND | − | − |
| C18 | + | + | + | + | − | ND | − | − |
| NPC1 | + | + | + | + | + | ND | + | − |
| NPC2 | + | + | + | + | + | ND | + | − |
| NPC3 | + | ND | ND | ND | ND | ND | ND | ND |
| NPC4 | + | ND | ND | ND | ND | ND | ND | ND |
| NPC5 | + | ND | ND | ND | ND | ND | ND | ND |
| NPC6 | + | ND | ND | ND | ND | ND | ND | ND |
| NPC7 | + | ND | ND | ND | ND | ND | ND | ND |
| C15 DNA | ++ | ++ | ++ | ++ | ++ | ND | ++ | ++ |
| CC | − | − | − | − | − | ND | − | − |
| BL-1 | + | + | + | + | + | ND | − | − |
| BL-2 | ++ | + | + | + | + | ND | − | − |
| BL-3 | + | + | + | + | + | ND | − | − |
| BL-4 | + | + | + | + | + | ND | − | − |
| BL-5 | + | + | + | + | + | ND | − | − |
| BL-6 | + | ND | ND | ND | ND | ND | ND | ND |
| BL-7 | + | ND | ND | ND | ND | ND | ND | ND |
| BL-8 | + | ND | ND | ND | ND | ND | ND | ND |
| BL-9 | + | ND | ND | ND | ND | ND | ND | ND |
| BL-10 | + | ND | ND | ND | ND | ND | ND | ND |
| BL-11 | + | ND | ND | ND | ND | ND | ND | ND |
| BL-12 | + | ND | ND | ND | ND | ND | ND | ND |
| Daudi DNA | ++ | ++ | ++ | ++ | ++ | ND | ++ | ++ |
| B95-8 | − | − | − | − | − | − | ND | − |
| M-ABA (−) | + | + | + | + | + | (+) | ND | − |
| M-ABA (+) | ++ | − | − | − | − | − | ND | − |
| Raji (−) | ++ | + | + | + | − | − | ND | − |
| Raji (+) | ++ | + | + | + | − | − | ND | − |
| Daudi (−) | +++ | ++ | ++ | ++ | + | + | ND | − |
| Daudi (+) | +++ | ++ | ++ | ++ | + | + | ND | − |
| P3HR-1(−) | +++ | ++ | ++ | ++ | + | + | ND | − |
| P3HR-1(+) | +++ | ++ | ++ | ++ | + | + | ND | − |
| Daudi DNA | ++ | ++ | ++ | ++ | ++ | ++ | ND | ++ |
aData with Ia-6T + Ia-8B based on ethidium bromide stained gels, all other products confirmed by Southern blot hybridisation of RT-PCR products.
bfrom accession number AJ507799.
c+++, very strongly positive; ++, strongly positive; +, weakly positive; −, negative, as determined by hybridisation data under identical conditions, and ND (not done), respectively. Some data taken from (22,23).