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Steady-state fluorescence emission spectra at 25°C of 9AP (A), 15AP (B) and 21AP (C) in the absence (solid lines) and presence (dashed lines) of saturating concentrations of HXDV. The solution conditions were 10 mM EPPS (pH 7.5) and sufficient KCl to bring the total K+ concentration to 150 mM.
