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(A) Binding curves of HXDV to 15,21AP derived from fluorescence titration profiles acquired at 15, 25, 35 and 45°C. The solid lines reflect fits of the experimental data points with Equation (7). (B) van't Hoff plots {ln(K) versus 1/T} depicting the temperature dependence of the association constants for the binding of HXDV to its two target sites on 15,21AP. The solid lines reflect linear fits of the experimental data points with Equation (8).
