Table 3.

Thermodynamic parameters for the binding of HXDV to its two target sites on 15,21AP at 35°C

Site	ΔG35a (kcal/mol)	ΔHb (kcal/mol)	ΔSb (cal/mol • K)
1	−7.4 ± 0.1	+3.8 ± 2.1	+36.0 ± 7.0
2	−6.5 ± 0.1	+4.0 ± 0.7	+34.4 ± 2.1

^a Values of ΔG₃₅ were calculated from the appropriate K-values listed in Table 2 using Equation (9), with the indicated uncertainties reflecting the maximum possible errors in K as propagated through this equation

^b Values of ΔH and ΔS were determined from fits of the van't Hoff plots shown in Figure 8B using Equation (8). The indicated uncertainties reflect the standard deviations of the experimental data from the fitted lines