Table 4.

Osmolyte dependence of the association constants (K) for the binding of HXDV to 15,21AP at 35°C^a

Osmolyte	Osmolality (osm)	K1b (M−1)	K2b (M−1)
None	0	(1.9 ± 0.1) × 105	(4.3 ± 0.1) × 104
Ethylene glycol	2.0	(2.8 ± 0.2) × 105	(4.8 ± 0.3) × 104
Glycerol	2.0	(2.9 ± 0.2) × 105	(3.8 ± 0.2) × 104

^a Buffer conditions were as described in the footnote to Table 1.

^b Values of K were derived as described in the footnote to Table 2.