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. 2007 May 21;35(10):e78. doi: 10.1093/nar/gkm250

Figure 3.

Figure 3

Integration of the hLhcgr gene into the mLhcgr BAC by ALFIRE. Resulting recombinants were screened over the 5′and 3′ integration sites. The integrity of the BAC (hmLhcgr) was first checked by PCR of a fragment including exons 5 and 6 (in the middle of the inserted region) and by restriction enzyme digestion pattern. (a) hLhcgr gene integration into the area between the mouse BAC promoter and 3′ end; (b) five clones where further screened by PCR over the middle region (exons 5 and 6) of the 55 kb insert; (c) the same clones were checked for contamination by either the hLhcgr-BAC or the mLhcgr-BAC by PCR amplification of the 3′ regions and (d) enzymatic digestion of mLhcgr, hLhcgr and hmLhcgr BAC clones with XhoI/PacI.