Table 1.

Efficiency for in vitro and in vivo BAC fusion (insertion of long sequences) with ALFIRE

	Incubation with cTc (min)	Tested colonies (n)	Positive colonies (n)	Efficiency (%)
In vitro	–	32	2	6.3
In E. coli	0	32	0	0
	15	32	22	69
	20	32	16	50
	30	32	6	19