Table 1.

Expressed proteins with their predicted sizes

Variant	Size (kDa)
Twinklea wt	74
K421A, G575D
Twinklea ▵AA346–376 (▵346–376)	71
Twinkya	64
Twinklea ▵AA70–343 (▵70–343)	41
POLG1 wt	142
D198A, D890N, D1135A
POLG1 ▵CAG	141

All expressed proteins had the MycHis epitope tag and this tag was included in the calculation of size in kiloDaltons (kDa).

^aSize based on predicted processing at AA42.