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. 2007 May 3;35(10):3494–3503. doi: 10.1093/nar/gkm248

Figure 4.

Figure 4.

Phosphorimage of reverse transcriptase products of the radiolabeled primer complementary to residues 548–569 (E. coli numbering). C, U, A and G indicate (dideoxy-)sequencing lanes, wt. + CMC: CMC derivatized and alkali treated 16S rRNA purified from C. acetobutylicum bacteria. wt. − CMC: alkali treated 16S rRNA purified from C. acetobutylicum bacteria. In vitro: T7 RNA polymerase transcribed 16S rRNA from the cloned 16S rRNA gene. Arrows indicate bands corresponding to modified nucleosides or secondary structure.