Figure 2.
(A) Interaction of CspB with ss DNA templates as monitored by ITC. Titration data for 27 injections of 5 μl of each oligonucleotide (100 μM) into a cell containing 5 μM CspB. Oligonucleotides used for ITC are listed below. All data have been corrected for heats of dilution. (B) Thermodynamic parameters for CspB-ss DNA interactions. Non-detectable = Kd ≤ 5 μM. (C) A molecular model of a potential cytosine-binding pocket on CspB. The electrostatic surface potential of CspB and the backbone trace of the bound consensus heptanucleotide and the hydrogen bonds (depicted as dotted lines) between CspB and a cytosine nucleobase in the proposed binding pocket.