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. 2007 Apr 25;35(10):3287–3296. doi: 10.1093/nar/gkm202

Figure 2.

Figure 2.

HPLC and electrophoretic analysis of purified 110mer RNA. (A) A PLRP-S 300 Å HPLC reverse-phase column was operated at a flow rate of 1 ml/min and maintained at a temperature of 80°C. The solvent system was buffers A and B, and the RNA was eluted with a linear gradient from 0 to 50% buffer B in 20 min. (B) A DNAPac PA100 HPLC anion-exchange column was operated at a flow rate of 1.5 ml/min and maintained at a temperature of 70°C. The solvent system was buffers C and D, and the RNA was eluted with a linear gradient from 5 to 50% buffer D in 20 min. (C) Capillary gel electrophoresis. (D) Polyacrylamide gel electrophoresis. The synthetic RNA was analyzed on a 5% polyacrylamide gel and stained with the cyanine dye SYBR Green II.