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. 2007 Apr 25;35(10):3287–3296. doi: 10.1093/nar/gkm202

Figure 6.

Figure 6.

Gene-silencing effect of chemically synthesized 110mer RNA. (A) Effect of 110mer RNA on expression of luciferase target gene. G3T-hi cells were transfected with pHOXB-Luc reporter plasmid and effector RNA (100 nM) and reporter assays performed 48 h after transfection. Each value shown is the average of three independent experiments, and standard deviations are indicated as error bars. (B) Comparison of sense/antisense suppression activity ratio for 110mer RNA. G3T-hi cells were transfected with pHOXB-Luc or pHOXB-Luc-antisense reporter plasmid and effector RNA (30 nM) and reporter assays performed 48 h after transfection. The results are presented as the ratio of the average of percent suppression of luciferase-containing sense and antisense target by 110mer RNA (pre-miRNA) or 22mer mature miRNA. (C) Sequences of the effector RNAs used. The underlined part of the 110mer pre-miR-196a-2 and the synthetic duplex 22mer miR-196a represent the sequence of the mature miRNA-196a.