Table 2.

Sequences and masses of fragments produced by RNase T1 digestion

			3′-Phosphate			2′,3′-Cyclic phosphate
Entry numbera	Sequence (5′→3′)	Peak	Calculated mass	Observed mass	Peak	Calculated mass	Observed mass
1	UG	A	670.4	N.I.b	A′	652.4	N.I.
2	CUCG	B	1280.8	1280.9	B′	1262.8	1262.9
3	CUCAG	C	1610.0	1610.2	C′	1592.0	N.D.c
4	CUG	D	975.6	975.6	D′	957.6	957.6
5	AUCUG	E	1611.0	1611.2	E′	1593.0	1593.2
6	CUUAG	E	1611.0	1611.2	E′	1593.0	1593.2
7	UAG	F	999.6	999.7	F′	981.6	N.D.
8	UUUCAUG	G	2223.3	2223.6	G′	2205.3	2205.6
9	UUG	H	976.6	N.I.	H′	958.6	N.I.
10	AUUG	I	1305.8	1306.0	I′	1287.8	1287.9
11	AG	J	693.5	N.I.	J′	675.5	N.I.
12	UUUUG	K	1588.9	1589.1	K′	1570.9	N.D.
13	AACUCG	L	1939.2	1939.5	L′	1921.2	1921.5
14	CAACAAG	M	2291.4	2291.8	M′	2273.4	2273.7
15	AAACUG	N	1963.2	1963.5	N′	1945.2	1945.5
16	CCUG	B	1280.8	1280.9	B′	1262.8	1262.9
17	UUACAUCAG	O	2880.7	2881.1	O′	2862.7	N.D.
18	UCG	D	975.6	975.6	D′	957.6	957.6
19	UUUUCG	P	1894.1	1894.4	P′	1876.1	1876.4

^aThe entry numbers correspond to the numbered fragments shown in Figure 4B.

^bN.I., not identified.

^cN.D., not detected.