Table 3.

Enthalpies and entropies for beacon stems, capture probes and detection probes in addition to kinetic data for the wild-type target

Name	ΔHstem	ΔSstem	ΔHdet	ΔSdet	ΔHcap	ΔScap	ΔHpen	ΔSpen	Rate
MB 5	−70.03	−0.2030	−93.43	−0.2596	NA	NA	NA	NA	1000.1
MB 6	−72.20	−0.2070	−85.44	−0.2373	NA	NA	NA	NA	562.4
MB 7	−76.13	−0.2161	−83.27	−0.2323	NA	NA	NA	NA	362.4
MB 8	−84.60	−0.2392	−76.56	−0.2118	NA	NA	NA	NA	219.3
MB 9	−93.91	−0.2639	−70.26	−0.1947	NA	NA	NA	NA	205.0
TP 5	−56.59	−0.1654	−93.43	−0.2596	−175.80	−0.4940	69.32	0.1993	96 233.9
TP 6	−62.42	−0.1804	−85.44	−0.2373	−175.80	−0.4940	67.49	0.1948	79 300.5
TP 7	−76.69	−0.2188	−83.27	−0.2323	−175.80	−0.4940	57.28	0.1657	72 428.2
TP 8	−78.78	−0.2237	−76.56	−0.2118	−175.80	−0.4940	52.33	0.1500	58 422.9
TP 9	−91.02	−0.2568	−70.26	−0.1947	−175.80	−0.4940	39.79	0.1129	55 861.1

Units are in kcal mol⁻¹ for enthalpic parameters, kcal mol⁻¹ K⁻¹ for entropic values and in M⁻¹ s⁻¹ for rate constants. TP detection probes were assumed to have the same thermodynamic parameters as the molecular beacons. Sign convention is for hairpin closing then binding with target.