Figure 2.

Recombination activities of attB mutant sites. Recombination activities are shown for the wild-type attB site (A), mutant sites at position 2 (B), 6 (C), 12 (D), 15 (E), 16 (F) 18 (G). Panel H shows the activities of partially symmetrized attB sites that contain the right sequence between +12 and +18 changed to the same sequence as on the left (−12 to −18), 2L (+12 to +18) or vice versa, 2R (−12 to −18). Recombination assays were performed using the standard plasmid assay containing the plasmid indicated in each panel and pRT702 encoding attP. The concentrations of integrase used for each set of six reactions in panels A to C and E, F and H was 0, 441, 110, 55, 27 and 14 nM. The concentrations of integrase used for each set of six reactions in panels D and G was 0, 351, 87, 43, 21 and 10 nM.