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. 2007 Jun 8;3(6):e94. doi: 10.1371/journal.pgen.0030094

Figure 5. Some GREs Are Conserved Nongenic Sequences That Exhibit Increased DNAse I Accessibility Upon GR Occupancy.

Figure 5

(A) Dex induces increased DNAse I accessibility. Nuclei from A549 cells treated with EtOH or dex for 1 h were isolated, treated with DNAse I, and harvested for DNA. The relative amount of the DNA at the corresponding region were assessed by qPCR and presented as percent cleavage, with standard error of mean averaged among at least three independent experiments. Controls #1 and #2 correspond to regions near AMOTL2 and CDH17 genes, respectively, which do not exhibit dex-induced GR occupancy (unpublished data).

(B) Human–mouse sequence conservation within GREs is presented. The mouse sequences aligned with 500-bp human GRE sequences were obtained from UCSC Genome Browser. The lengths of the continuous GRE sequences without gaps between human and mouse are shown, and the percent identity of these regions were calculated as number of matched base pairs divided by length of fragment.