Figure 4. Feeding Persistence and Transmission of Leishmania to Multiple Hosts.

Flies were fed blood alone (open square), L. infantum (dotted square), L. mexicana stationary phase (shaded square), or exponential phase amastigotes (closed square). The number of flies feeding on a different host when interrupted was determined on days 5, 7, and 10 post-infection/feeding in a behavioral assay. n = 12 per point, representing the combination of two independent experiments. Error bars 1 s.e.m. Asterisks indicate values that are statistically significant (*p < 0.05, **p < 0.005) from blood fed control flies using an unpaired t-test.