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. 2007 Jun 29;3(6):e92. doi: 10.1371/journal.ppat.0030092

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© 2007 Xu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) CaFT profiles of nocodazole, benomyl, mebendazole and thiabendazole. Note that the ScFT experiments with these compounds have not been reported. Highlighted, in addition to TUB1 (orf19.7308) and TUB2 (orf19.6034), are BUB1 (orf19.2678), MAD2 (orf19.1040) and ESP1 (orf19.3356) strains that showed modest but reproducible hypersensitivity to nocodazole. The corresponding proteins are involved in mitotic checkpoint regulation and spindle pole body assembly [27]. Their relevance becomes apparent in the results described in Figure 7.

(B) Spot tests of heterozygous deletion strains highlighted in (A) against benomyl. Note the lack of hypersensitivity of TUB2 in spot tests is consistent with the CaFT results, implying that the failure to detect hypersensitivity of the TUB2 strain in the CaFT is not due to poor performance of the barcodes.

(C) TUB1 and TUB2 strains are haploinsufficient under the standard growth conditions (see Figure 3 legend for experimental details).