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PCR primers and parameters
| PCR | Primer | Sequence | [Mg] mM | Anneal temp. | Amplicon size (bp) | T melt Celsius |
| Bos taurus | cytb F | 5'-CCATCGAACATTTCATCATGATGGAA-3' | 2.0 | 64 | 359 | 95 |
| cytb R | 5'-GCTCCTCAGAATGATATTTGTCCTCA-3' | |||||
| IS1081 | F2 | 5'-CTGCTCTCGACGTTCATCGCCG-3' | 135 | 94 | ||
| R2 | 5'-GGCACGGGTGTCGAAATCACG-3' | 2.0 | 58 | 113 | ||
| R3 | 5-TGGCGGTAGCCGTTGCGC-3 | |||||
| RD4 flanking | F1 | 5'-AATGGTTTGGTCATGACGCCTTC-3' | 58 | 176 | 92 | |
| F2 | 5-TGTGAATTCATACAAGCCGTAGTC-3 | 2.0 | 142 | |||
| R1 | 5'-CCCGTAGCGTTACTGAGAAATTGC-3' |
