Table 1.
List of proteins co-expressed without and with chaperone combinations 1 to 5 and effects of chaperones on yields of purified, soluble target proteins, applying either the one-step or two-step procedure.
| Protein | MW (kDa) | Increased solubility by | |||
| one step procedure | two step procedure | ||||
| Yield increase | Best chaperone combination | Yield increase | Best chaperone combination | ||
| 1Fringe | 43 | ∞ | 4 | 3 | 4 |
| 2Ap | 54 | ∞ | 4 | 3 | 3 |
| 22f21 | 52 | ∞ | 3 | 3 | 4 |
| LZip | 41 | ∞ | 1 | n.d. | n.d. |
| Oskar3 | 118 | ∞ | 2/4 | 2.5 | 2 |
| 2c18 | 50 | 3 | 4 | 8 | 4 |
| 2Tep4 | 68 | 3.5 | 4 | 13 | 4 |
| BtKe | 55 | 7 | 3 | 42 | 4 |
| PhK | 29 | 3 | 4 | 7 | 5 |
| Tep1 | 8 | 3 | 5 | 6 | 1 |
| Susy | 86 | 2.5 | 3 | 5 | 5 |
| Xklp3A+Xklp3B | 20+ 16 | 2.5 | 4 | 3.5 | 4 |
| B1R | 59 | 3.5 | 3 | 3.5 | 3 |
| E8R2 | 85 | 5.5 | 3 | 5.5 | 3 |
| GTR1 | 35 | 3 | 4 | 3 | 4 |
| HbpH | 9 | 3.5 | 4 | 3.5 | 4 |
| Rolled | 43 | 4.5 | 3 | 4.5 | 3 |
| Xklp3A4 | 44 | 2.5 | 5 | 2.5 | 5 |
| Xklp3B3 | 70 | 2.5 | 4 | 2.5 | 4 |
| Mash+ Susy | 90+ 86 | 3 | 4 | 3 | 4 |
| 1Tep3 | 47 | 4 | 3 | n.d. | n.d. |
| 1Tep4 | 45 | 3.5 | 5 | n.d. | n.d. |
| BtKc | 64 | 3 | 5 | n.d. | n.d. |
| E8R1 | 58 | 5 | 5 | n.d. | n.d. |
| MaxF | 7.5 | 3 | 4 | n.d. | n.d. |
| Tev prot. | 30 | 3.5 | 3 | n.d. | n.d. |
| Xklp3A1 | 62 | 1* | - | ∞ | 2 |
| Xklp3B1 | 40 | 1* | - | ∞ | 5 |
| 2Fringe | 55 | 1 | - | 2 | 4 |
| Xklp3A5 | 35 | 1 | - | 19 | 4 |
| 2Tep3 | 70 | 1 | - | 11 | 2 |
| BtKp | 55 | 1 | - | 28 | 4 |
| Mash | 91 | 1 | - | 3 | 2 |
| PPAT | 22 | 1 | - | 3 | 4 |
| Eg5 | 95 | 1 | - | 1 | - |
| F10L | 72 | 1 | - | 1 | - |
| Tep2 | 11 | 1 | - | 1 | - |
| 1AP | 52 | 1 | - | n.d. | n.d. |
| Chip | 64 | 1 | - | n.d. | n.d. |
| dLMO | 37 | 1 | - | n.d. | n.d. |
| Pex5P | 38 | 1 | - | n.d. | n.d. |
| Endostatin | 42 | 1* | - | 1* | - |
| Kringle | 30 | 1* | - | 1* | - |
| Lzip2 | 37 | 1* | - | 1* | - |
| UCP1 | 33 | 1* | - | 1* | - |
| Tlc4 | 57 | 1* | - | n.d. | n.d. |
| Xklp3A2 | 38 | 1* | - | n.d. | n.d. |
| Xklp3A3 | 72 | 1* | - | n.d. | n.d. |
| Xklp3B1 | 35 | 1* | - | n.d. | n.d. |
| Xpot1 | 108 | 1* | - | n.d. | n.d. |
The increase in solubility of target proteins relative to control cells without co-overexpression of chaperones (set as 1) is given in the column "yield increase". The chaperone combinations for optimal yield of each target protein in the one-step and two-step procedures are indicated. Proteins that exhibited solubility exclusively after chaperone co-overexpression are indicated by "∞". The symbol "*" indicates that no soluble protein was purified from cells with or without chaperone co-overproduction. n.d., not determined. The same protein name can appear more than one time in the list because different constructs (domains or fusions) have been used for its expression.