. Author manuscript; available in PMC: 2007 Jul 2.

Published in final edited form as: J Cell Sci. 2005 Oct 1;118(Pt 19):4527–4539. doi: 10.1242/jcs.02568

Table 1.

Quantification of the co-localisation of GFP-SNX2 with various endosomal markers

	Percentage of structures
Marker	Co-localisation	GFP-SNX2 alone	Marker alone
EEA1	70.3±5.6	16.0±6.0	11.7±2.3
EGFR, 10 min	71.0±5.9	12.3±3.4	17.7±9.3
SNX1	78.4±6.4	9.5±7.7	12.1±1.3
LAMP1	9.0±4.2	49.5±12.0	41.5±7.8

HeLa cells expressing low levels of GFP-SNX2 were fixed and stained for the various markers. For co-localisation with internalised EGF receptor, serum-starved cells were stimulated for 10 minutes with EGF (100 ng/ml) prior to fixation. The degree of co-localisation was determined as described in Materials and Methods.