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. Author manuscript; available in PMC: 2007 Oct 1.
Published in final edited form as: Cell Immunol. 2007 Feb 26;243(2):96–106. doi: 10.1016/j.cellimm.2007.01.003

Figure 1. Neonatal monocytes isolated from fresh cord blood are deficient at MHC-II antigen processing and presentation.

Figure 1

Negatively selected monocytes isolated from fresh HLA-matched cord and adult blood was incubated for 24 h with antigen and appropriate HLA-restricted T hybridoma cells. Supernatants were assessed for IL-2 content by a CTLL-2 proliferation assay that was monitored with Alamar blue, an indicator dye. A & B) HLA-DR1 (allele DRB1*0101) neonatal and adult monocytes incubated with tetanus toxoid and ITIA T hybridoma cells. C) HLA-DR4 (allele DRB1*0401) neonatal and adult monocytes incubated with tetanus toxoid and ITC T hybridoma cells. Data points are means of triplicate samples +/− S.D.