Table II.
cAMP responsiveness of cells expressing FLAG-LHR-wt and FLAG-LHR-D578H1
| Cell Line | Treatment | Fold Increase over basal cAMP levels | n |
|---|---|---|---|
| LHR-wt | None | 1.0a | 9 |
| LHR-wt | 100 nM hCG | 1.6 ± 0.1b | 9 |
| LHR-D578H | None | 2.1 ± 0.3b | 9 |
| LHR-D578H | 1% MβCD | 2.9 ± 0.6b | 6 |
CHO cells expressing LHR-wt were assayed for cAMP expression following treatment with 100 nM hCG or following pretreatment of cells expressing LHR-D578H with MβCD. Results are expressed as the mean of the relative increase in cAMP ± S.E.M. compared to basal levels of cAMP in untreated CHO cells expressing LHR-wt receptors.
Means with different superscripts differ (p<0.03).
Means with different superscripts differ (p<0.03).