Table III.
Tracking of individual anti-FLAG-gold particles bound to LHR-wt or LHR-D578H on CHO cells.
| Cell line | Hormone | Number of particles analyzed | Number of domains/ 2 min trajectory | D0-1a (10-11cm2sec-1) | D = Lr2/4tb (10-11cm2sec-1) | Timec (sec) | Domain Diameter(Lr)c |
|---|---|---|---|---|---|---|---|
| LHR-wt | none | 8 | 4 ± 1 | 5.1 ± 2.1 | 1.3 ± 1.1 | 27 ± 9 | 309 ± 131 |
| LHR-wt | hCG | 8 | 4 ± 1 | 1.7 ± 0.8 | 0.3 ± 0.1 | 25 ± 11 | 146 ± 19 |
| LHR-D578H | none | 8 | 4 ± 1 | 0.7 ± 0.3 | 0.2 ± 0.1 | 23 ± 11 | 114 ± 22 |
| LHR-D578H | hCG | 8 | 4 ± 1 | 0.7 ± 0.1 | 0.1 ± 0.1 | 19 ± 8 | 94 ± 33 |
a
D0-1: Diffusion coefficient of the first 2 points [19]
b
D presents the diffusion coefficient within a domain as described by Saxton [20].
c
The average diameter of an individual domain was calculated as described by Daumas et al. [19] and Murase et al. [18].
d
Average time for residence within a domain