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. 2007 Jun 27;405(Pt 2):351–357. doi: 10.1042/BJ20061737

Figure 1. SPR-based analyses of the binding of IX-bp to Factors IX and X.

Figure 1

The activated CM4 chip was coated with 20 μg/ml IX-bp, by injecting 60 μl of the solution at a flow rate of 10 μl/min, and was blocked with 1 M ethanolamine hydrochloride at a similar flow rate for 7 min. Then 40 μl aliquots (100 nM final concentration) of factors were injected at a flow rate of 20 μl/min for 2 min. Appropriate concentrations of calcium or magnesium ions were added to the buffer (10 mM Hepes, pH 7.4, and 150 mM NaCl). FIX, Factor IX.